Lindqvist L, Nord C E, Sôder P O
Enzyme. 1977;22(3):166-75. doi: 10.1159/000458785.
Whole saliva of 59 healthy persons was used for determination of esterase activity. The pattern of esterase was studied by means of isoelectrofocusing on thin-layer acrylamide gels. The esterases found in whole saliva are suggested to be derived from the cells of the tissue in the oral cavity. This origin is indicated (e.g.) by comparison between isolectrophoretic esterase patterns of whole saliva, submandibular saliva, gingival biopsy and fibroblast culture. Antisera against partially purified saliva esterases were produced in rabbits. These sera, used in immunoelectro-osmophoresis, gave esterase-active immunoprecipitate against whole saliva, the water-soluble materials of disrupted gingival biopsy and fibroblast culture, but not against the material of the bacteria, Streptococcus sanguis, Strptococcus mutans, Streptococcus mitis, Actinomyces viscosus and Lactobacillus fermentum.
采用59名健康人的全唾液来测定酯酶活性。通过在薄层丙烯酰胺凝胶上进行等电聚焦研究酯酶模式。全唾液中发现的酯酶被认为源自口腔组织细胞。（例如）通过比较全唾液、颌下唾液、牙龈活检组织和成纤维细胞培养物的等电电泳酯酶模式表明了这种来源。在兔体内制备了针对部分纯化唾液酯酶的抗血清。这些血清用于免疫电渗电泳，可产生针对全唾液、破碎牙龈活检组织和成纤维细胞培养物的水溶性物质的酯酶活性免疫沉淀物，但不针对血链球菌、变形链球菌、缓症链球菌、黏性放线菌和发酵乳杆菌的物质。
