Tagu D, Nasse B, Martin F
I.N.R.A, Centre de Recherches Forestières, Equipe de Microbiologie Forestière, Seichamps, France.
Gene. 1996 Feb 2;168(1):93-7. doi: 10.1016/0378-1119(95)00725-3.
Major alterations of fungal gene expression are induced by the development of ectomycorrhiza, a symbiosis between tree roots and filamentous fungi. Several cDNAs corresponding to highly expressed transcripts of the Basidomycete Pisolithus tinctorius (Pt) were isolated from symbiotic tissues. Two of these abundant transcripts (hydPt-1 and hydPt-2) encoded polypeptides belonging to the hydrophobin (Hyd) family, a group of small cysteine-rich fungal proteins involved im morphogenesis and plant-fungus interactions. As shown for other Hyd, the hydPt-1 and hydPt-2 mRNAs were barely detectable in mycelium grown in liquid culture and highly accumulated in aerial hyphae. In addition, these transcripts were also abundant in eucalyptus globulus-Pt ectomycorrhiza in early stages of differentiation, during the colonisation of roots.
外生菌根（一种树根与丝状真菌之间的共生关系）的形成会诱导真菌基因表达发生重大改变。从共生组织中分离出了几个与担子菌彩色豆马勃（Pt）高表达转录本相对应的cDNA。其中两个丰富的转录本（hydPt - 1和hydPt - 2）编码属于疏水蛋白（Hyd）家族的多肽，这是一组富含半胱氨酸的小型真菌蛋白，参与形态发生和植物 - 真菌相互作用。正如其他Hyd蛋白所示，hydPt - 1和hydPt - 2 mRNA在液体培养的菌丝体中几乎检测不到，而在气生菌丝中高度积累。此外，在根定植期间的分化早期，这些转录本在蓝桉 - Pt外生菌根中也很丰富。
