Lewin M R, Wyllie J H
Eur Surg Res. 1977;9(3):166-72. doi: 10.1159/000127936.
Inactivation of gastrin was studied (a) in vitro by incubation with a high-speed supernatant fraction of rat small bowel mucosa and (b) in vivo by perfusing gastrin through the small bowel vascular bed in anaesthetized dogs. In both types of experiment there was a highly significant loss in the bioactivity of gastrin, but no significant change in its immunoreactivity. This showed that gastrin was inactivated by a subtle chemical change which rendered the molecule biologically inactive, yet left its immunoreactivity unimpaired.
（a）在体外，将其与大鼠小肠黏膜的高速上清液部分一起孵育；（b）在体内，通过在麻醉犬的小肠血管床灌注胃泌素进行研究。在这两种实验类型中，胃泌素的生物活性均有高度显著的丧失，但其免疫反应性无显著变化。这表明胃泌素是通过一种微妙的化学变化而失活的，这种变化使分子失去生物活性，但未损害其免疫反应性。
