Whitley P, Nilsson I M, von Heijne G
Department of Biochemistry, Stockholm University, Sweden.
J Biol Chem. 1996 Mar 15;271(11):6241-4. doi: 10.1074/jbc.271.11.6241.
We have measured the minimum number of residues in a translocating polypeptide required to bridge the distance between the P-site in endoplasmic reticulum-bound ribosomes and the lumenally disposed active site of the oligosaccharyl transferase. The results suggest that a nascent chain may traverse the ribosome/translocase complex in a largely extended conformation, and that hydrophobic stop-transfer segments have a more compact, possibly alpha-helical conformation in the translocase.
我们已经测量了转运多肽中跨越内质网结合核糖体的P位点与腔面寡糖基转移酶活性位点之间距离所需的最小残基数。结果表明，新生肽链可能以大体伸展的构象穿过核糖体/转位酶复合物，并且疏水的停止转移片段在转位酶中具有更紧密的构象，可能是α-螺旋构象。
