Conder G A, Johnson S S
Upjohn Laboratories, Kalamazoo, Michigan 49001, USA.
J Parasitol. 1996 Feb;82(1):100-2.
Various techniques were examined to determine optimum conditions for exsheathing infective larvae of 3 important ruminant parasites (Haemonchus contortus, Ostertagia ostertagi, and Trichostrongylus colubriformis). In repeated experiments, aliquots of 10(5)-10(6) infective larvae, 1-2 mo old, of each parasite were incubated in each of 4 exsheathing media (distilled water, Earle's balanced salt solution + carbon dioxide, nematode washing buffer + carbon dioxide, or sodium hypochlorite) for 1 or 18 hr. In each case, the percentage of larvae exsheathed and infectivity for jirds was determined. Results of these studies indicate that no single exsheathing technique of those studied is optimum for every parasite. In addition, caution must be used in drawing conclusions from in vitro studies using exsheathed larvae because techniques that routinely provide high percentages of exsheathment also appear to reduce viability.
研究了多种技术，以确定3种重要反刍动物寄生虫（捻转血矛线虫、奥斯特他线虫和哥伦比亚细颈线虫）感染性幼虫脱鞘的最佳条件。在重复实验中，将每种寄生虫1 - 2月龄的10⁵ - 10⁶个感染性幼虫等分试样，分别置于4种脱鞘培养基（蒸馏水、Earle平衡盐溶液 + 二氧化碳、线虫洗涤缓冲液 + 二氧化碳或次氯酸钠）中孵育1小时或18小时。每种情况下，均测定幼虫脱鞘的百分比以及对沙鼠的感染性。这些研究结果表明，所研究的脱鞘技术中，没有一种对每种寄生虫都是最佳的。此外，在使用脱鞘幼虫进行体外研究得出结论时必须谨慎，因为常规能提供高脱鞘率的技术似乎也会降低活力。
