Bosanquet A G, Bell P B
Bath Cancer Research Unit, University of Bath, Royal United Hospital, U.K.
Leuk Res. 1996 Feb;20(2):143-53. doi: 10.1016/0145-2126(95)00127-1.
Ex vivo drug sensitivity testing is of considerable benefit in aiding the choice of optimum chemotherapy for leukaemia patients, especially when several therapeutic options exist, e.g. for relapsed chronic lymphocytic leukaemia (CLL). We have used the Differential Staining Cytotoxicity (DiSC) assay to assess drug sensitivity in CLL for over a decade and here present many methodological improvements, including depositing multiple samples per microscope slide and performing a rapid LC90 evaluation. Using these improvements, 412/450 specimens were successfully tested. Failures were mainly due to extended specimen transit time. All 38 drugs tested exhibited dose-dependent cell kill and broad ranges of resultant LC90S were observed. Comparison of 2- and 4-day incubations underscored a requirement for 4-day incubation with pentostatin and steroids. The rapid, simple and streamlined DiSC assay presented here can aid choice of optimum therapy, identify novel anticancer agents and be used to study drug resistance.
体外药敏试验对于帮助白血病患者选择最佳化疗方案具有相当大的益处，尤其是在存在多种治疗选择时，例如复发性慢性淋巴细胞白血病（CLL）。我们使用差异染色细胞毒性（DiSC）试验评估CLL的药物敏感性已有十多年，在此展示了许多方法上的改进，包括在每张显微镜载玻片上放置多个样本以及进行快速LC90评估。利用这些改进，成功检测了450个样本中的412个。失败主要是由于样本运输时间延长。所测试的所有38种药物均表现出剂量依赖性细胞杀伤作用，并且观察到了广泛的LC90结果范围。对2天和4天孵育的比较强调了喷司他丁和类固醇需要进行4天孵育。这里介绍的快速、简单且简化的DiSC试验有助于选择最佳治疗方案、识别新型抗癌药物并用于研究耐药性。
