Shirley D M, Williams S A, Santos P M
Division of Otolaryngology, Southern Illinois University, Springfield, 62794, USA.
Laryngoscope. 1996 May;106(5 Pt 1):629-32. doi: 10.1097/00005537-199605000-00021.
The potential benefit of brain-derived neurotrophic factor (BDNF) on motor-nerve regeneration after transection injury in 24 adult rats was evaluated after entubulation repair. Gait analysis for ankle angle and tension transduction device (TTD) strain-gauge measurements yielded functional evaluation of regeneration. The BDNF (15 mg/mL) or phosphate buffered saline (control) was injected into the silicone elastomer (Silastic) channel. Gait analysis performed 0, 2, 4, 6, 10, and 12 weeks after injury demonstrated a significant difference between uninjured and injured legs of 23 and 43 degrees, respectively (P<.001, analysis of variance). The TTD evaluation 13 weeks after injury demonstrated a significant decrease in force development of injured compared to uninjured legs, 148 and 58 g, respectively (P<.001). No functional benefits were demonstrated between BDNF-treated versus control-treated animals in either model for a single exposure to BDNF.
在24只成年大鼠进行插管修复后，评估了脑源性神经营养因子（BDNF）对横断损伤后运动神经再生的潜在益处。通过对踝关节角度的步态分析和张力转导装置（TTD）应变计测量对再生进行功能评估。将BDNF（15 mg/mL）或磷酸盐缓冲盐水（对照）注入硅橡胶通道。在损伤后0、2、4、6、10和12周进行的步态分析表明，未受伤腿和受伤腿之间分别有23度和43度的显著差异（P<.001，方差分析）。损伤后13周的TTD评估表明，受伤腿与未受伤腿相比，力量发展显著降低，分别为148克和58克（P<.001）。在单次暴露于BDNF的两种模型中，BDNF治疗组和对照组动物之间均未显示出功能益处。
