通过转导高水平链霉素抗性来恢复苜蓿中华根瘤菌无效突变体的有效性。
Restoration of effectiveness of R. meliloti ineffective mutants by transduction of high level streptomycin resistance.
作者信息
Zelazna-Kowalska I, Kowalski M
出版信息
Acta Microbiol Pol. 1978;27(4):339-45.
PMID:86281
Abstract
Rhizobium meliloti lysine dependent mutant, L5-30lys, was ineffective and this mutation was not cotransducible to lys. Transduction of chloramphenicol and linked temperature sensitive mutations did not change its symbiotic properties. Subsequent transduction of high level streptomycin resistance restored its effectiveness. Streptomycin resistance marker was linked to chloramphenicol resistance and temperature sensitive markers.
摘要
苜蓿中华根瘤菌赖氨酸依赖型突变体L5 - 30lys是无效的，并且该突变不能与lys共转导。氯霉素转导及相关的温度敏感突变并未改变其共生特性。随后高水平链霉素抗性的转导恢复了其有效性。链霉素抗性标记与氯霉素抗性及温度敏感标记相关联。
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