Gorman S W, Banasiak D, Fairley C, McCormick S
Plant Gene Expression Center, USDA/ARS, Albany, CA USA.
Mol Gen Genet. 1996 Apr 24;251(1):52-9. doi: 10.1007/BF02174344.
Pollen development requires both sporophytic and gametophytic gene expression. We are using a map-based cloning technique to isolate sporophytic genes which, when mutant, cause pollen abortion and a male sterile (ms) phenotype in tomato (Lycopersicon esculentum). We have genetically characterized one ms locus (ms14) using RFLP analysis and identified flanking markers. High-resolution genomic physical mapping indicates that the ms14 locus is located in a approximately 300 kb region. We have identified a YAC clone with an insert size of approximately 610 kb that contains the ms14-linked markers, reflects the organization of the physical map and therefore most probably contains the ms14 gene. In addition, we present evidence that the relationship between physical and genetic distance in this chromosomal region changes abruptly from approximately 105-140 kb/cM to less than 24kb/cM, and suggest that the TG393-TG104 region is a hotspot for recombination.
花粉发育需要孢子体和配子体基因表达。我们正在使用基于图谱的克隆技术来分离孢子体基因，这些基因在发生突变时会导致番茄（Lycopersicon esculentum）花粉败育和雄性不育（ms）表型。我们利用RFLP分析对一个ms位点（ms14）进行了遗传特征分析，并鉴定了侧翼标记。高分辨率基因组物理图谱表明，ms14位点位于大约300 kb的区域内。我们鉴定出一个插入片段大小约为610 kb的YAC克隆，它包含与ms14连锁的标记，反映了物理图谱的结构，因此很可能包含ms14基因。此外，我们提供的证据表明，该染色体区域物理距离与遗传距离之间的关系从大约105 - 140 kb/cM突然变为小于24 kb/cM，并表明TG393 - TG104区域是重组热点。
