Rad23对于酿酒酵母中的转录偶联修复和高效的整体修复是必需的。
Rad23 is required for transcription-coupled repair and efficient overrall repair in Saccharomyces cerevisiae.
作者信息
Mueller J P, Smerdon M J
机构信息
Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164-4660, USA.
出版信息
Mol Cell Biol. 1996 May;16(5):2361-8. doi: 10.1128/MCB.16.5.2361.
Abstract
The repair of UV-induced photoproducts (cyclobutane pyrimidine dimers) in a well-characterized minichromosome, genomic DNA, and a transcribed genomic gene (RPB2) of a rad23delta mutant of Saccharomyces care was examined. Isogenic wild-type cells show a strong bias for the repair of the transcribed strands in both the plasmid and genomic genes and efficient overall repair of both DNAs (>80% of the dimers were removed in 6 h). However, the rad23delta mutant shows (i) no strand bias for repair in these genes and decreased repair of both strands, (ii) partial repair of genomic DNA (approximately 45% in 6 h), and (iii) very poor repair of the plasmid overall approximately 15% in 6 h). These features, coupled with the decreased UV survival of rad23delta cells, indicate that Rad23 is required for both transcription-coupled repair and efficient overall repair in S. cerevisiae.
摘要
对酿酒酵母rad23δ突变体的一个特征明确的微型染色体、基因组DNA和一个转录的基因组基因（RPB2）中紫外线诱导的光产物（环丁烷嘧啶二聚体）的修复情况进行了检测。同基因野生型细胞在质粒和基因组基因中对转录链的修复均表现出强烈偏好，并且对两种DNA都有高效的整体修复（6小时内>80%的二聚体被去除）。然而，rad23δ突变体表现出：（i）在这些基因中修复无链偏好且两条链的修复均减少；（ii）基因组DNA的部分修复（6小时内约45%）；（iii）质粒的整体修复非常差（6小时内约15%）。这些特征，再加上rad23δ细胞紫外线存活率的降低，表明Rad23是酿酒酵母转录偶联修复和高效整体修复所必需的。
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本文引用的文献
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Inducible removal of UV-induced pyrimidine dimers from transcriptionally active and inactive genes of Saccharomyces cerevisiae.从酿酒酵母转录活性和非活性基因中诱导去除紫外线诱导的嘧啶二聚体。
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Mechanisms of transcription-repair coupling and mutation frequency decline.转录修复偶联机制与突变频率下降
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