一种由与U5 snRNA互补的2'-O-甲基寡核糖核苷酸诱导形成的U1/U4/U5 snRNP复合物。
A U1/U4/U5 snRNP complex induced by a 2'-O-methyl-oligoribonucleotide complementary to U5 snRNA.
作者信息
Ast G, Weiner A M
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
出版信息
Science. 1996 May 10;272(5263):881-4. doi: 10.1126/science.272.5263.881.
Nuclear messenger RNA splicing involves multiple interactions between the five spliceosomal small nuclear ribonucleoprotein particles (snRNPs) U1, U2, U4, U5, and U6 and numerous spliceosomal proteins. Here it is shown that binding of a 2'-O-methyl-oligoribonucleotide complementary to U5 small nuclear RNA (snRNA) nucleotides 68 to 88 (BU5Ae) disrupts the initial U4/U5/U6 tri-snRNP complex, enhances the U2/U6 interaction, and induces a Ul/U4/U5 snRNP complex. The Ul/U4/U5 snRNP complex interacts specifically with an RNA oligonucleotide containing the 5' splice site sequence and may therefore represent a transitional stage in the displacement of U1 from the 5' splice site by U5 snRNP.
核信使核糖核酸剪接涉及5种剪接体小核核糖核蛋白颗粒（snRNP）U1、U2、U4、U5和U6与众多剪接体蛋白之间的多种相互作用。本文表明，与U5小核RNA（snRNA）核苷酸68至88互补的2'-O-甲基寡核糖核苷酸（BU5Ae）的结合会破坏最初的U4/U5/U6三snRNP复合物，增强U2/U6相互作用，并诱导形成U1/U4/U5 snRNP复合物。U1/U4/U5 snRNP复合物与含有5'剪接位点序列的RNA寡核苷酸特异性相互作用，因此可能代表U5 snRNP将U1从5'剪接位点置换过程中的一个过渡阶段。
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