Rafi A, Feval F
UCL Medical School, Department of Medical Microbiology, London, England.
Southeast Asian J Trop Med Public Health. 1995 Jun;26(2):253-7.
In this study of leprosy patients with putative tuberculosis, the polymerase chain reaction (PCR), one of the most reliable and sensitive molecular diagnostic methods, was carried out for the specific detection of Mycobacterium tuberculosis DNA. Sputum samples from 43 patients at Baba Baghi Leprosarium in Iran were tested. The DNA extraction method was based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate (GuSCN) together with the nucleic acid binding properties of diatoms or silica particles. Primers for a 123-base pair (bp) fragment of the repetitive DNA sequence of M. tuberculosis were used for the PCR assay. The results of PCR were compared with direct microscopy and culture. In total, 14% of the patients in this study were found to be PCR positive for M. tuberculosis. No positive results were found by direct microscopy for acid fast bacilli (AFB) and culture. It was thought probable that the positive PCR results indicated the tuberculosis (TB) in such treated leprosy patients.
在这项针对疑似结核病的麻风病患者的研究中，采用了聚合酶链反应（PCR）这一最可靠、最灵敏的分子诊断方法之一，对结核分枝杆菌DNA进行特异性检测。对来自伊朗巴巴巴吉麻风病院的43例患者的痰液样本进行了检测。DNA提取方法基于硫氰酸胍（GuSCN）的裂解和核酸酶灭活特性以及硅藻或二氧化硅颗粒的核酸结合特性。用于PCR检测的引物是针对结核分枝杆菌重复DNA序列的123个碱基对（bp）片段。将PCR结果与直接显微镜检查和培养结果进行了比较。在这项研究中，总共发现14%的患者结核分枝杆菌PCR检测呈阳性。直接显微镜检查未发现抗酸杆菌（AFB）阳性结果，培养也未发现阳性结果。据认为，PCR阳性结果可能表明此类接受治疗的麻风病患者患有结核病（TB）。
