用于检测乳腺肿瘤细针穿刺活检中erbB-2基因扩增的荧光原位杂交技术。
Fluorescence in situ hybridization for detecting erbB-2 amplification in breast tumor fine needle aspiration biopsies.
作者信息
Sauter G, Feichter G, Torhorst J, Moch H, Novotna H, Wagner U, Dürmüller U, Waldman F M
机构信息
Department of Pathology, University of Basel, Switzerland.
出版信息
Acta Cytol. 1996 Mar-Apr;40(2):164-73. doi: 10.1159/000333683.
OBJECTIVE
To evaluate the feasibility of erbB-2 amplification analysis of fine needle aspiration (FNA) biopsies.
STUDY DESIGN
FNA smears and dissociated nuclei from 58 breast cancer samples were examined by dual-labeling fluorescence in situ hybridization (FISH) with probes for centromere 17 and the erbB-2 gene. The results were compared with the outcome of erbB-2 immunohistochemistry.
RESULTS
Tumors were categorized according to the erbB-2/centromere 17 signal ratio. There were 23 tumors with high-level amplification, four cases with a low-level erbB-2 gain and 27 tumors with normal erbB-2 content. Four tumors showed an erbB-2 deletion, all in patients < or = 42 years of age. ErbB-2 amplification was strongly associated with positive erbB-2 immunostaining (P < .0001). Comparison of FISH analysis on dissociated cells and on FNA biopsies showed high correspondence (P < .0001).
CONCLUSION
FISH allows reliable detection of erbB-2 gene amplification on FNA biopsies.
目的
评估细针穿刺（FNA）活检标本中erbB-2基因扩增分析的可行性。
研究设计
采用针对17号染色体着丝粒和erbB-2基因的探针，通过双标记荧光原位杂交（FISH）技术对58例乳腺癌样本的FNA涂片及解离后的细胞核进行检测。将结果与erbB-2免疫组化结果进行比较。
结果
根据erbB-2/17号染色体着丝粒信号比值对肿瘤进行分类。有23例肿瘤为高水平扩增，4例为低水平erbB-2增加，27例肿瘤erbB-2含量正常。4例肿瘤显示erbB-2缺失，所有患者年龄均≤42岁。erbB-2扩增与erbB-2免疫染色阳性密切相关（P<.0001）。解离细胞与FNA活检标本的FISH分析结果具有高度一致性（P<.0001）。
结论
FISH能够可靠地检测FNA活检标本中的erbB-2基因扩增。
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