Lee M, Cheung H T
Department of Biological Sciences, Illinois State University, Normal 61790-4120, USA.
Biochem Biophys Res Commun. 1996 Apr 25;221(3):503-9. doi: 10.1006/bbrc.1996.0626.
The extracellular matrix (ECM) plays an important structural and functional role in multicellular organisms. Because of the similarities between C. elegans and vertebrate development, this nematode could serve as a simplified model to study the biology of the ECM. In this study, a method for extracting mammalian ECM was adapted for the extraction of C. elegans ECM. ECM components from C. elegans were found to be homologous to mammalian ECM by immunoblotting. It was also demonstrated that antibodies generated against C. elegans ECM stained basement membrane-like structures in C. elegans eggs, larvae, and adults.
细胞外基质（ECM）在多细胞生物中发挥着重要的结构和功能作用。由于秀丽隐杆线虫与脊椎动物发育存在相似性，这种线虫可作为研究ECM生物学的简化模型。在本研究中，一种提取哺乳动物ECM的方法被改编用于提取秀丽隐杆线虫的ECM。通过免疫印迹发现，秀丽隐杆线虫的ECM成分与哺乳动物的ECM同源。还证明了针对秀丽隐杆线虫ECM产生的抗体可对秀丽隐杆线虫卵、幼虫和成虫中的基底膜样结构进行染色。
