Tamura M, Nagaoka S, Kawagoe M
Department of Molecular Pharmacology, Tokyo Medical and Dental University, Japan.
J Endod. 1996 May;22(5):240-3. doi: 10.1016/s0099-2399(06)80140-8.
Interstitial collagenase (matrix metalloprotease-1) is a member of a family of matrix metalloproteases and is thought to play a role in degradation of the extracellular components, such as collagens in normal extracellular matrix remodeling and in many disease processes. We examined interstitial collagenase mRNA expression in human dental pulp fibroblast cultures by Northern blot analysis. These cells did not express interstitial collagenase mRNA in an unstimulated condition. Inflammatory cytokines, such as interleukin-1 alpha, induced interstitial collagenase mRNA expression in these cells. The interstitial collagenase mRNA levels began to increase after 2-h exposure, reaching a maximum after 8 h, then dropping to the unstimulated level at 48 h. These effects were observed in a dose-dependent manner in a dose range of 0.1 to 10 ng/ml. Transforming growth factor-beta reduced the levels of interstitial collagenase mRNA expression that were induced by interleukin-1 alpha. These observations suggest that interstitial collagenase mRNA expression in human dental pulp fibroblasts is regulated by the inflammatory cytokines and that interstitial collagenase may play a role in tissue degradation in inflamed dental pulp.
间质胶原酶（基质金属蛋白酶-1）是基质金属蛋白酶家族的一员，被认为在细胞外成分的降解中发挥作用，例如在正常细胞外基质重塑以及许多疾病过程中的胶原蛋白降解。我们通过Northern印迹分析检测了人牙髓成纤维细胞培养物中间质胶原酶mRNA的表达。这些细胞在未受刺激的条件下不表达间质胶原酶mRNA。炎性细胞因子，如白细胞介素-1α，可诱导这些细胞中间质胶原酶mRNA的表达。间质胶原酶mRNA水平在暴露2小时后开始升高，8小时后达到最大值，然后在48小时降至未受刺激水平。在0.1至10 ng/ml的剂量范围内，这些效应呈剂量依赖性。转化生长因子-β降低了白细胞介素-1α诱导的间质胶原酶mRNA表达水平。这些观察结果表明，人牙髓成纤维细胞中间质胶原酶mRNA的表达受炎性细胞因子调节，并且间质胶原酶可能在炎症牙髓的组织降解中发挥作用。
