通过近场扫描光学显微镜检测人类中期染色体上的荧光原位杂交。

Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy.

作者信息

Moers M H, Kalle W H, Ruiter A G, Wiegant J C, Raap A K, Greve J, de Grooth B G, van Hulst N F

机构信息

Department of Applied Physics, University of Twente, AE Enschede, The Netherlands.

出版信息

J Microsc. 1996 Apr;182(Pt 1):40-5. doi: 10.1111/j.1365-2818.1996.tb04795.x.

DOI:10.1111/j.1365-2818.1996.tb04795.x

PMID:8632445

Abstract

Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.

摘要

通过近场扫描光学显微镜检测人类中期染色体上的荧光原位杂交。这种细胞化学和扫描探针技术的结合能够以前所未有的分辨率在单条染色体上定位和识别几个荧光标记的基因组DNA片段。使用了三种核酸探针：pUC1.77、p1 - 79和质粒探针α - 血影蛋白。杂交信号在近场荧光图像中得到了很好的分辨，而探针的确切位置可以与剪切力图像提供的染色体形貌精确关联。