Stoffel M, Le Beau M M, Espinosa R, Bohlander S F, Le Paslier D, Cohen D, Xiang K S, Cox N J, Fajans S S, Bell G I
Howard Hughes Medical Institute, University of Chicago, IL 60637, USA.
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3937-41. doi: 10.1073/pnas.93.9.3937.
We have generated a physical map of human chromosome bands 20q11.2-20q13.1, a region containing a gene involved in the development of one form of early-onset, non-insulin-dependent diabetes mellitus, MODY1, as well as a putative myeloid tumor suppressor gene. The yeast artificial chromosome contig consists of 71 clones onto which 71 markers, including 20 genes, 5 expressed sequence tags, 32 simple tandem repeat DNA polymorphisms, and 14 sequence-tagged sites have been ordered. This region spans about 18 Mb, which represents about 40% of the physical length of 20q. Using this physical map, we have refined the location of MODY1 to a 13-centimorgan interval (approximately equal to 7 Mb) between D20S169 and D20S176. The myeloid tumor suppressor gene was localized to an 18-centimorgan interval (approximately equal to 13 Mb) between RPN2 and D20S17. This physical map will facilitate the isolation of MODY1 and the myeloid tumor suppressor gene.
我们构建了人类染色体20q11.2 - 20q13.1区域的物理图谱，该区域包含一个与早发型非胰岛素依赖型糖尿病的一种类型（MODY1）的发生相关的基因，以及一个假定的髓系肿瘤抑制基因。酵母人工染色体重叠群由71个克隆组成，71个标记已在这些克隆上进行了排序，其中包括20个基因、5个表达序列标签、32个简单串联重复DNA多态性以及14个序列标签位点。该区域跨度约18 Mb，约占20号染色体物理长度的40%。利用这一物理图谱，我们已将MODY1的定位精确到D20S169和D20S176之间13厘摩的区间（约等于7 Mb）。髓系肿瘤抑制基因定位于RPN2和D20S17之间18厘摩的区间（约等于13 Mb）。这一物理图谱将有助于分离MODY1和髓系肿瘤抑制基因。
