Bortvin A, Winston F
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
Science. 1996 Jun 7;272(5267):1473-6. doi: 10.1126/science.272.5267.1473.
Genetic analysis has implicated SPT6, an essential gene of Saccharomyces cerevisiae, in the control of chromatin structure. Mutations in SPT6 and particular mutations in histone genes are able to overcome transcriptional defects in strains lacking the Snf/Swi protein complex. Here it is shown that an spt6 mutation causes changes in chromatin structure in vivo. In addition, both in vivo and in vitro experiments provide evidence that Spt6p interacts directly with histones and primarily with histone H3. Consistent with these findings, Spt6p is capable of nucleosome assembly in vitro.
遗传分析表明，酿酒酵母的必需基因SPT6参与染色质结构的调控。SPT6中的突变以及组蛋白基因中的特定突变能够克服缺乏Snf/Swi蛋白复合物的菌株中的转录缺陷。本文表明，spt6突变在体内会导致染色质结构发生变化。此外，体内和体外实验均提供证据表明，Spt6p直接与组蛋白相互作用，且主要与组蛋白H3相互作用。与这些发现一致，Spt6p在体外能够进行核小体组装。
