通过大肠杆菌转化鉴定多形汉逊酵母重组基因组释放的多个质粒。
Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli.
作者信息
Graupner S, Wackernagel W
机构信息
Fachbereich Biologie, Universität Oldenburg, Germany.
出版信息
Appl Environ Microbiol. 1996 May;62(5):1839-41. doi: 10.1128/aem.62.5.1839-1841.1996.
Abstract
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.
摘要
从两株多形汉逊酵母（季也蒙毕赤酵母）中分离得到的总DNA，这两株酵母的染色体上存在源自pUC18的表达质粒的单拷贝或串联多拷贝整合，这些总DNA产生了大肠杆菌转化子，这些转化子所含质粒的大小和/或结构与表达质粒不同。本研究提供了证据，证明在多形汉逊酵母中形成了质粒样结构，并且DNA损伤会刺激其形成。
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