Herrmann M, Zocher R, Haese A
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Federal Republic of Germany.
Mol Plant Microbe Interact. 1996 May;9(4):226-32. doi: 10.1094/mpmi-9-0226.
Production of the phytotoxic compound enniatin has been proposed to play a role during the infection process of plants by enniatin-synthesizing Fusarium species. Enniatins are cyclohexadepsipeptides synthesized by the multifunctional enzyme enniatin synthetase. To test the hypothesis that enniatin contributes to pathogenicity, enniatin-nonproducing mutants were constructed by gene disruption of the enniatin synthetase gene of a virulent Fusarium avenaceum strain. Four independent enniatin nonproducing mutants were characterized that did not express enniatin synthetase, as proved by RNA and protein blot analysis. The virulence on potato tuber tissue of the enniatin-nonproducing strains was significantly reduced compared with the virulence of the parent strain and three enniatin-producing transformants. Therefore, we conclude that enniatin production contributes to the virulence of Fusarium avenaceum.
有人提出，植物毒性化合物恩镰孢菌素的产生在合成恩镰孢菌素的镰刀菌属物种感染植物的过程中发挥作用。恩镰孢菌素是由多功能酶恩镰孢菌素合成酶合成的环六肽缩肽。为了验证恩镰孢菌素有助于致病性这一假设，通过对一株致病的燕麦镰刀菌菌株的恩镰孢菌素合成酶基因进行基因破坏，构建了不产生恩镰孢菌素的突变体。通过RNA和蛋白质印迹分析证明，鉴定出四个独立的不产生恩镰孢菌素的突变体，它们不表达恩镰孢菌素合成酶。与亲本菌株和三个产生恩镰孢菌素的转化体的毒力相比，不产生恩镰孢菌素的菌株对马铃薯块茎组织的毒力显著降低。因此，我们得出结论，恩镰孢菌素的产生有助于燕麦镰刀菌的毒力。
