Sydenham E W, Shephard G S, Thiel P G, Stockenström S, Snijman P W, Van Schalkwyk D J
Medical Research Council, Programme on Mycotoxins and Experimental Carcinogenesis, Tygerberg, South Africa.
J AOAC Int. 1996 May-Jun;79(3):688-96.
A liquid chromatographic (LC) method for simultaneous determination of fumonisins B1 (FB1), B2 (FB2), and B3 (FB3) in corn was subjected to a collaborative study involving 12 participants from 10 countries, in which the accuracy and reproducibility characteristics of the method were established. Mean analyte recoveries from corn ranged from 81.1 to 84.2% for FB1 (at a spiking range of 500 to 8000 ng/g), from 75.9 to 81.9% for FB2 (at a spiking range of 200 to 3200 ng/g), and from 75.8 to 86.8% for FB3 (at a spiking range of 100 to 1600 ng/g). The valid data were statistically evaluated after exclusion of outliers. Relative standard deviations for within-laboratory repeatability ranged from 5.8 to 13.2% for FB1, from 7.2 to 17.5% for FB2, and from 8.0 to 17.2% for FB3. Relative standard deviations for between-laboratory reproducibility varied from 13.9 to 22.2% for FB1, from 15.8 to 26.7% for FB2, and from 19.5 to 24.9% for FB3. HORRAT ratios, calculated for the individual toxin analogues, ranged from 0.75 to 1.73. The LC method for determination of fumonisins B1, B2, and B3 in corn (at concentrations of 800-12800 ng total fumonisins/g) has been adopted by AOAC INTERNATIONAL.
一种用于同时测定玉米中伏马毒素B1（FB1）、B2（FB2）和B3（FB3）的液相色谱（LC）方法进行了一项协同研究，该研究有来自10个国家的12名参与者，确立了该方法的准确性和重现性特征。玉米中各分析物的平均回收率为：FB1在加标范围为500至8000 ng/g时为81.1%至84.2%，FB2在加标范围为200至3200 ng/g时为75.9%至81.9%，FB3在加标范围为100至1600 ng/g时为75.8%至86.8%。排除异常值后对有效数据进行了统计评估。实验室内重复性的相对标准偏差为：FB1为5.8%至13.2%，FB2为7.2%至17.5%，FB3为8.0%至17.2%。实验室间重现性的相对标准偏差为：FB1为13.9%至22.2%，FB2为15.8%至26.7%，FB3为19.5%至24.9%。为各毒素类似物计算的HORRAT比值范围为0.75至1.73。AOAC INTERNATIONAL已采用该用于测定玉米中伏马毒素B1、B2和B3（总伏马毒素浓度为800 - 12800 ng/g）的LC方法。
