Uthe J F, Misra R K, King T L, Musial C J
Department of Fisheries and Oceans, Marine Environmental Sciences Division, Maritimes Region, Halifax, Canada.
J AOAC Int. 1996 May-Jun;79(3):797-802.
A method is presented for estimating replicate polycyclic aromatic hydrocarbon (PAH) concentrations in American lobster (Homarus americanus) digestive gland tissue based on recoveries of added perdeuterated surrogates from a single satisfactory analysis. PAH concentrations demonstrated a large interanimal variance, even in specimens captured at the same time in the same place. Principal component analysis showed that the variability of the total system of biological variables (carapace length, lobster weight, and digestive gland weight) could be adequately summarized by the first principal component alone in each data set. Ranks provide ordered classification of individuals, allowing data analysis by statistical methods for continuous variables (i.e., analysis of variance). PAH concentrations in individual lobsters were generally highly sensitive to animal size, sex, and fishing area. Efficient monitoring would result from analyzing individual animals of a single sex from a study area, using as small a geographical study area as possible, measuring a single biological variable, and using individual specimens of as narrow a size range as possible.
本文提出了一种基于单次满意分析中添加的全氘代替代物的回收率来估算美洲龙虾（美洲螯龙虾）消化腺组织中多环芳烃（PAH）重复浓度的方法。即使在同一时间、同一地点捕获的样本中，PAH浓度也表现出较大的个体间差异。主成分分析表明，在每个数据集中，仅第一个主成分就可以充分概括生物变量（甲壳长度、龙虾重量和消化腺重量）整个系统的变异性。秩提供了个体的有序分类，允许使用连续变量的统计方法进行数据分析（即方差分析）。个体龙虾中的PAH浓度通常对动物大小、性别和捕捞区域高度敏感。通过分析来自研究区域的单一性别的个体动物，使用尽可能小的地理研究区域，测量单一生物变量，并使用尽可能窄的大小范围的个体样本，可以实现高效监测。
