Bianchi V, Donzelli G
Vincenza Bianchi Laboratory of Clinical Pathology, City Hospital, Alessandria, Italy.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):162-7. doi: 10.1016/0378-4347(95)00330-4.
The main active cannabis (marijuana and hashish) derivative delta 9-tetrahydrocannabinol is, in vivo, transformed and excreted mainly as 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) and its glucuronide. The method presented here allows the confirmation of the presence of THC-COOH by means of a basic hydrolysis, solid-phase extraction clean-up on reversed-phase (RP) disposable cartridges followed by analysis on a C8 RP column and UV detection; the mobile phase used was a 55% acetonitrile solution in acid phosphate buffer. Over 600 samples both from drug addicts in therapeutic communities and subjects who were not on any drugs therapy were analysed. This method was precise with a linearity range from 10 to more than 500 ng/ml [the lower limit proposed by the National Institute on Drug Abuse (NIDA) for cannabinoid confirmation method is 15 ng/ml]. The sample preparation is simple and fast, allowing the analysis of large numbers of samples. Perfect correlation was observed between data from the HPLC method and a fluorescence polarization immunoassay screening method. The THC-COOH metabolite was found to constitute 30% of all the cannabinoids excreted in urine of abusers.
大麻（大麻和哈希什）的主要活性衍生物Δ9-四氢大麻酚在体内主要转化并以11-去甲-Δ9-四氢大麻酚-9-羧酸（THC-COOH）及其葡糖醛酸苷的形式排泄。本文介绍的方法通过碱性水解、在反相（RP）一次性柱上进行固相萃取净化，随后在C8 RP柱上分析并采用紫外检测，从而确认THC-COOH的存在；所用流动相为酸性磷酸盐缓冲液中55%的乙腈溶液。对来自治疗社区的吸毒者和未接受任何药物治疗的受试者的600多个样本进行了分析。该方法精确，线性范围为10至500 ng/ml以上[美国国立药物滥用研究所（NIDA）提出的大麻素确认方法的下限为15 ng/ml]。样品制备简单快速，可对大量样品进行分析。在高效液相色谱法数据与荧光偏振免疫分析筛查法数据之间观察到了完美的相关性。发现THC-COOH代谢物占滥用者尿液中排泄的所有大麻素的30%。
