采用荧光检测的灵敏高效液相色谱法测定长春瑞滨血药浓度。
Sensitive high-performance liquid chromatographic method with fluorescence detection for measurement of vinorelbine plasma concentrations.
作者信息
Robieux I, Vitali V, Aita P, Freschi A, Lazzarini R, Sorio R
机构信息
Laboratorio di Farmacologia Clinica, Centro di Riferimento Oncologico, Aviano, Italy.
出版信息
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):183-7. doi: 10.1016/0378-4347(95)00332-0.
A high-performance liquid chromatographic (HPLC) method with fluorescence detection for the determination of vinorelbine in plasma is described. The technique was derived from that published by Debal for an assay of vinorelbine in cell culture medium. The modifications concern the preparation procedure for plasma samples (a two-step liquid-liquid extraction from plasma is described), optimization of the mobile phase composition, and use of a single C18 column. These changes resulted in an improved sensitivity and reproducibility of the assay and led to its feasibility for clinical pharmacokinetic studies. The range of the assay is 2 to 1000 ng/ml.
描述了一种采用荧光检测的高效液相色谱（HPLC）法测定血浆中长春瑞滨的含量。该技术源自德巴尔发表的用于测定细胞培养基中长春瑞滨的方法。改进之处包括血浆样品的制备程序（描述了从血浆中进行两步液 - 液萃取的方法）、流动相组成的优化以及使用单一的C18柱。这些改变提高了测定的灵敏度和重现性，并使其适用于临床药代动力学研究。该测定的范围为2至1000 ng/ml。
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