Andrä S, Frey G, Nitsch M, Baumeister W, Stetter K O
Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.
FEBS Lett. 1996 Jan 29;379(2):127-31. doi: 10.1016/0014-5793(95)01493-4.
From Methanopyrus kandleri, the most thermophilic methanogen known so far, we have purified to homogeneity a protein complex of high molecular mass. Image analysis of transmission electron micrographs revealed a barrel-shaped particle composed of two rings with 8-fold symmetry. Only one type of subunit could be detected. The corresponding gene has been cloned and sequenced. The deduced amino acid sequence shows high homology with the members of group II chaperonins. The structure of the projection and the sequence homology suggest that this particle is the first thermosome isolated from a methanogen.
从目前已知嗜热程度最高的产甲烷菌——坎氏甲烷嗜热菌中，我们已将一种高分子量蛋白质复合物纯化至同质状态。透射电子显微镜图像分析显示，该复合物为桶状颗粒，由具有八重对称性的两个环组成。仅检测到一种亚基类型。相应基因已被克隆并测序。推导的氨基酸序列与第二组伴侣蛋白成员具有高度同源性。其投影结构和序列同源性表明，该颗粒是从产甲烷菌中分离出的首个热体。
