用于简化酵母基因破坏载体构建的可选择盒式结构。
Selectable cassettes for simplified construction of yeast gene disruption vectors.
作者信息
Earley M C, Crouse G F
机构信息
Graduate Program in Genetics and Molecular Biology, Emory University, Atlanta, GA 30322, USA.
出版信息
Gene. 1996 Feb 22;169(1):111-3. doi: 10.1016/0378-1119(95)00805-5.
PMID:8635733
Abstract
Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of an KmR-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the hisG repeats flanking the gene, permitting reuse of the URA3 marker.
摘要
基于hisG-URA3-hisG插入片段的盒式结构已通过添加编码卡那霉素抗性（KmR）的基因和侧翼多克隆位点进行了修饰，极大地简化了大肠杆菌中基因破坏载体的构建。在酵母中进行基因破坏后，URA3随后可通过基因两侧hisG重复序列之间的重组被切除，从而允许URA3标记的重复使用。
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