Berben G, Dumont J, Gilliquet V, Bolle P A, Hilger F
Unité de Microbiologie, Faculté des Sciences Agronomiques de Gembloux, Belgium.
Yeast. 1991 Jul;7(5):475-7. doi: 10.1002/yea.320070506.
The YDp plasmids (Yeast Disruption plasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used (HIS3, LEU2, LYS2, TRP1 or URA3). The markers, surrounded by translational termination codons, are embedded in the slightly modified sequence of the pUC9 multiple cloning sites.
YDp质粒(酵母破坏质粒)是携带一组酵母基因破坏盒的pUC9载体,其结构均一,仅在所用的选择标记(HIS3、LEU2、LYS2、TRP1或URA3)上有所不同。这些标记被翻译终止密码子包围,嵌入在pUC9多克隆位点的略微修饰的序列中。