鼠伤寒沙门氏菌atp突变体在碳饥饿期间HemA-LacZ杂合蛋白抗蛋白水解的稳定性
Stabilization of a HemA-LacZ hybrid protein against proteolysis during carbon starvation in atp mutants of Salmonella typhimurium.
作者信息
Archer C D, Jin J, Elliott T
机构信息
Department of Microbiology and Immunology, West Virginia University Health Sciences Center, Morgantown 26506, USA.
出版信息
J Bacteriol. 1996 Apr;178(8):2462-4. doi: 10.1128/jb.178.8.2462-2464.1996.
Abstract
Transposon insertions that stabilize the beta-galactosidase activity of a HemA-LacZ hybrid protein following carbon starvation were mapped to the atp operon of Salmonella typhimurium. This effect is similar to that seen with nuo mutants defective in the energy-conserving type I NADH dehydrogenase. Insertions in several other genes, including such highly pleiotropic mutants as rpoS, polA, and hfq, were isolated with the same phenotypic screen, but they do not affect the beta-galactosidase activity of HemA-LacZ. All of these mutants act indirectly to alter the colony color of many different fusion strains on indicator plates.
摘要
碳饥饿后能稳定HemA-LacZ杂合蛋白β-半乳糖苷酶活性的转座子插入位点被定位到鼠伤寒沙门氏菌的atp操纵子上。这种效应与能量保守型I NADH脱氢酶缺陷的nuo突变体所观察到的效应相似。通过相同的表型筛选分离出了其他几个基因中的插入突变体，包括rpoS、polA和hfq等多效性很强的突变体，但它们并不影响HemA-LacZ的β-半乳糖苷酶活性。所有这些突变体都间接影响指示平板上许多不同融合菌株的菌落颜色。
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