Kinetics of sperm penetration and fertilization in vitro in hamster follicular and oviductal ova.

This study compared kinetics of in vitro sperm penetration and fertilization of in vivo matured follicular vs. oviductal hamster ova. Monospermic fertilization in cumulus-intact (C+) and cumulus-free (C-) follicular ova was highest at sperm concentrations of approximately 50 and 1.0 x 10(4) sperm/ml, respectively. The cumulus layer was a barrier to sperm penetration, but penetration of C-follicular ova was still lower than with C-oviductal ova. At 1 h of sperm:egg coincubation, 21% of C- follicular ova was penetrated vs. 77% of C- oviductal ova, while by 4 h these values were 71% and 98%, respectively. In contrast, activation of zona-free follicular and oviductal ova was not different (83-99%). However, at 2 h male pronuclear formation was lower in follicular ova (9%) than in oviductal ova (26%, P < 0.05). To test the hypothesis that lower and slower penetration with follicular ova compared with oviductal ova is due to lack of oviductal exposure, effects of hamster periovulatory oviductal fluid on penetration and fertilization kinetics of C- follicular ova were examined. Adding oviductal fluid did not improve penetration of follicular ova to levels equivalent to oviductal ova, but 3 h preincubation of follicular ova with oviductal fluid improved both zona penetration and male pronucleus formation to levels equivalent to those in oviductal ova. We conclude that kinetics of sperm penetration and fertilization in follicular ova are retarded, that these deficiencies derive from lack of ovum modulation by oviductal secretions, and that prolonged oviductal exposure of ova is essential for achieving full competence.