Hernandez-Munoz H E, Stanford J L
Department of Bacteriology, University College London Medical School, UK.
J Med Microbiol. 1996 Mar;44(3):165-9. doi: 10.1099/00222615-44-3-165.
IgA and IgG antibodies to cytoplasmic and secreted antigens of serotypes 4 and 8 of Mycobacterium avium and the percentage of agalactosyl immunoglobulin (%Gal[0]) were measured by ELISA in groups of blood donors, HIV seronegative persons, HIV seropositive persons with CD4+ cell counts >300/mm3 and AIDS patients co-infected with M.avium. No differences were found between the control groups, but HIV seropositive persons were distinguished by their increased %Gal[0] (p<0.001) and increased IgA titre (p<0.05) to secreted antigens of both serotypes of M. avium. Patients going on to develop aviumosis differed from other HIV-positive individuals, having more IgA to secreted antigens of serotype 8 (p<0.03) and more IgG to secreted antigens of both serotypes (p<0.0001), IgA titres fell to both types of antigen from serotype 4 (p<0.01) and sonicate antigen of serotype 8 (p<0.001) and IgG fell to the secreted antigens of serotype 4 (p<0.03). On interpretation of these observations that antibody profiles to M. avium might be used to identify healthy persons at special risk of developing HIV seropositivity, and to identify persons with early AIDS who are likely to develop aviumosis.
采用酶联免疫吸附测定法（ELISA）检测了献血者、HIV血清学阴性者、CD4+细胞计数>300/mm3的HIV血清学阳性者以及合并鸟分枝杆菌感染的艾滋病患者体内针对鸟分枝杆菌4型和8型胞质及分泌抗原的IgA和IgG抗体，以及无半乳糖基免疫球蛋白的百分比（%Gal[0]）。对照组之间未发现差异，但HIV血清学阳性者的特点是%Gal[0]升高（p<0.001）以及针对鸟分枝杆菌两种血清型分泌抗原的IgA滴度升高（p<0.05）。进展为鸟分枝杆菌病的患者与其他HIV阳性个体不同，其针对8型分泌抗原的IgA更多（p<0.03），针对两种血清型分泌抗原的IgG更多（p<0.0001），针对4型抗原和8型超声处理抗原的IgA滴度均下降（p<0.01），针对4型分泌抗原的IgG下降（p<0.03）。基于这些观察结果可以推断，针对鸟分枝杆菌的抗体谱可能有助于识别有发生HIV血清学阳性特殊风险的健康人，以及识别可能进展为鸟分枝杆菌病的早期艾滋病患者。
