全反式维甲酸联合α干扰素可有效抑制慢性粒细胞白血病中的粒细胞-巨噬细胞集落形成。

All-trans retinoic acid combined with interferon-alpha effectively inhibits granulocyte-macrophage colony formation in chronic myeloid leukemia.

作者信息

Zheng A, Savolainen E R, Koistinen P

机构信息

Department of Internal Medicine, University of Oulu, FInland.

出版信息

Leuk Res. 1996 Mar;20(3):243-8. doi: 10.1016/0145-2126(95)00148-4.

DOI:10.1016/0145-2126(95)00148-4

PMID:8637219

Abstract

We investigated the effect of all-trans retinoic acid (ATRA) alone and in combination with interferon-alpha (IFN-alpha) on the granulocyte-macrophage (GM) colony formation of peripheral blood progenitors isolated from patients with chronic myeloid leukemia (CML) (n = 12) or other myeloproliferative disorders (n = 10) as well as from healthy controls (n = 7). The ATRA or IFN-alpha alone inhibited slightly, but not significantly, the GM colony growth in CML. Granulocyte-macrophage colony formation decreased significantly (P<0.05) when ATRA and IFN-alpha were combined (114 +/- 96 versus 74 +/- 53 colonies/10(4) mononuclear cells). The combination did not have any inhibitory effect on the other MPDs. In healthy controls, ATRA or IFN-alpha alone or their combination stimulated GM colony growth, the increase being from 22 +/- 9 to 39 +/- 16 colonies for ATRA (P<0.05), up to 47 +/- 12 colonies for IFN-alpha (P<0.05) and up to 50 +/- 19 colonies for the combination (P<0.05). In conclusion, ATRA combined with IFN-alpha inhibits GM colony growth in CML. This combination may be worth testing clinically as a treatment of CML.

摘要

我们研究了全反式维甲酸（ATRA）单独使用以及与α干扰素（IFN-α）联合使用对从慢性粒细胞白血病（CML）患者（n = 12）、其他骨髓增殖性疾病患者（n = 10）以及健康对照者（n = 7）分离出的外周血祖细胞的粒细胞-巨噬细胞（GM）集落形成的影响。单独使用ATRA或IFN-α对CML患者的GM集落生长有轻微抑制作用，但不显著。当ATRA与IFN-α联合使用时，粒细胞-巨噬细胞集落形成显著减少（P<0.05）（114±96对74±53个集落/10⁴个单核细胞）。该联合用药对其他骨髓增殖性疾病没有任何抑制作用。在健康对照者中，单独使用ATRA或IFN-α或它们联合使用均刺激GM集落生长，ATRA组从22±9个集落增加到39±16个集落（P<0.05），IFN-α组增加到47±12个集落（P<0.05），联合用药组增加到50±19个集落（P<0.05）。总之，ATRA与IFN-α联合使用可抑制CML患者的GM集落生长。这种联合用药可能值得作为CML的一种治疗方法进行临床测试。