Gundersen C B, Umbach J A, Mastrogiacomo A
Department of Molecular and Medical Pharmacology, UCLA School of Medicine 90095, USA.
Life Sci. 1996;58(22):2037-40. doi: 10.1016/0024-3205(96)00195-6.
We used tunicamycin, an inhibitor of protein fatty acylation, to examine the possibility that there is a cycle of acylation and deacylation of cysteine string proteins at nerve terminals. Using both physiological and immunoblot approaches, we obtained no evidence for a cycle of acylation and deacylation that affects these proteins. These data suggest that this lipid modification of cysteine string proteins is relatively more stable than that observed for other nerve ending proteins, like SNAP-25.
我们使用衣霉素（一种蛋白质脂肪酰化抑制剂）来研究神经末梢处半胱氨酸串珠蛋白存在酰化和去酰化循环的可能性。通过生理学和免疫印迹方法，我们没有获得影响这些蛋白质的酰化和去酰化循环的证据。这些数据表明，半胱氨酸串珠蛋白的这种脂质修饰相对比其他神经末梢蛋白（如SNAP-25）所观察到的更稳定。
