Lindberg L A, Palkama A
Histochem J. 1977 May;9(3):277-84. doi: 10.1007/BF01004763.
The effect of fixation with a bicarbonate-buffered solution of paraformaldehyde and polyvinyl pyrrolidone (PVP) on the ultrastructural demonstration of glycogen and phosphorylase activity in rat hepatocytes has been studied. Phosphorylase was demonstrated by the precipitation of liberated phosphate ions with ferrous ions. 7.5% PVP was included in all steps in the procedure before post-fixaiton in osmium tetroxide. Glycogen particles were well preserved. Structures connecting membranes and glycogen particles were also evident. Phosphorylase activity was rapidly inhibited by the fixative; the fixation time was, therefore, kept very short. The final reaction product was localized on glycogen particles and on endoplasmic membranes in association with glycogen particles. The results support the view that endoplasmic membranes are involved in the metabolism of glycogen in hepatocytes.
研究了用多聚甲醛和聚乙烯吡咯烷酮（PVP）的碳酸氢盐缓冲溶液固定对大鼠肝细胞糖原和磷酸化酶活性超微结构显示的影响。通过亚铁离子沉淀游离磷酸根离子来显示磷酸化酶。在四氧化锇后固定之前的所有步骤中都加入7.5%的PVP。糖原颗粒保存良好。连接膜和糖原颗粒的结构也很明显。固定剂能迅速抑制磷酸化酶活性；因此，固定时间保持非常短。最终反应产物定位于糖原颗粒以及与糖原颗粒相关的内质膜上。这些结果支持内质膜参与肝细胞糖原代谢的观点。
