Källberg E, Jainandunsing S, Gray D, Leanderson T
Immunology Unit, Lund University, Sweden.
Science. 1996 Mar 1;271(5253):1285-9. doi: 10.1126/science.271.5253.1285.
The molecular mechanism behind affinity maturation is the introduction of point mutations in immunoglobulin (Ig) V genes, followed by the selective proliferation of B cells expressing mutants with increased affinity for antigen. An in vitro culture system was developed in which somatic hypermutation of Ig V genes was sustained in primed B cells. Cognate T cell help and cross-linking of the surface Ig were required, whereas the addition of lipopolysaccharide or a CD40 ligand to drive proliferation was insufficient. This system should facilitate understanding of the molecular and cellular mechanisms that regulate somatic mutation and B cell selection.
亲和力成熟背后的分子机制是免疫球蛋白（Ig）V基因中引入点突变，随后表达对抗原亲和力增加的突变体的B细胞选择性增殖。开发了一种体外培养系统，其中Ig V基因的体细胞超突变在致敏B细胞中得以维持。需要同源T细胞辅助和表面Ig的交联，而添加脂多糖或CD40配体来驱动增殖是不够的。该系统应有助于理解调节体细胞突变和B细胞选择的分子和细胞机制。
