Shibuya H, Yamaguchi K, Shirakabe K, Tonegawa A, Gotoh Y, Ueno N, Irie K, Nishida E, Matsumoto K
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Science. 1996 May 24;272(5265):1179-82. doi: 10.1126/science.272.5265.1179.
Transforming growth factor-beta (TGF-beta) regulates many aspects of cellular function. A member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family, TAK1, was previously identified as a mediator in the signaling pathway of TGF-beta superfamily members. The yeast two-hybrid system has now revealed two human proteins, termed TAB1 and TAB2 (for TAK1 binding protein), that interact with TAK1. TAB1 and TAK1 were co-immunoprecipitated from mammalian cells. Overproduction of TAB1 enhanced activity of the plasminogen activator inhibitor 1 gene promoter, which is regulated by TGF-beta, and increased the kinase activity of TAK1. TAB1 may function as an activator of the TAK1 MAPKKK in TGF-beta signal transduction.
转化生长因子-β（TGF-β）调节细胞功能的多个方面。促分裂原活化蛋白激酶激酶激酶（MAPKKK）家族成员TAK1，先前被确定为TGF-β超家族成员信号通路中的一种介质。酵母双杂交系统现已揭示出两种与TAK1相互作用的人类蛋白质，称为TAB1和TAB2（TAK1结合蛋白）。TAB1和TAK1从哺乳动物细胞中共免疫沉淀出来。TAB1的过量表达增强了受TGF-β调节的纤溶酶原激活物抑制剂1基因启动子的活性，并增加了TAK1的激酶活性。TAB1可能在TGF-β信号转导中作为TAK1 MAPKKK的激活剂发挥作用。
