Verdier F, Aujoulat M, Condevaux F, Descotes J
Pharmakon Europe, L'Arbresle, France.
Toxicology. 1995 Dec 20;105(1):81-90. doi: 10.1016/0300-483x(95)03127-2.
Lymphocyte subset counts and cytokine assays are useful to investigate the interactions of pharmaceuticals, particularly new biotechnology products, with the immune system. As no specific reagents are available to label monkey lymphocytes or to assay monkey cytokines by ELISA, cross reactivities of a panel of monoclonal antibodies specific for human lymphocytes or cytokines were studied in the Cynomolgus monkey. The proportions of B, T, CD4+ and CD8+ cells were determined by flow cytometry using a whole blood technique with at least one monoclonal antibody for each subset. Background data were obtained for more than 300 samples. Monkey and human cultured white blood cells were stimulated with standard mitogens. PHA + LPS in humans and Con A + PWM in monkeys triggered the greatest proliferation. IL-1 beta IL-2, IL-6, IL-8, TNF-alpha, TNF-beta and IFN-gamma, but not IL-1 alpha, were detected in the monkey using human reagents. In addition, the cytokine profile and the kinetics of cytokine production compared well in humans and Cynomolgus monkeys.
淋巴细胞亚群计数和细胞因子检测对于研究药物，尤其是新型生物技术产品与免疫系统的相互作用很有用。由于没有可用于标记猴淋巴细胞或通过酶联免疫吸附测定法（ELISA）检测猴细胞因子的特异性试剂，因此研究了一组针对人淋巴细胞或细胞因子的单克隆抗体在食蟹猴中的交叉反应性。使用全血技术通过流式细胞术测定B细胞、T细胞、CD4 +和CD8 +细胞的比例，每个亚群至少使用一种单克隆抗体。获得了300多个样本的背景数据。用标准有丝分裂原刺激猴和人培养的白细胞。人用PHA + LPS，猴用Con A + PWM引发最大增殖。使用人试剂在猴中检测到IL-1β、IL-2、IL-6、IL-8、TNF-α、TNF-β和IFN-γ，但未检测到IL-1α。此外，人和食蟹猴的细胞因子谱和细胞因子产生动力学比较相似。
