Beer B, Scherer J, zur Megede J, Norley S, Baier M, Kurth R
Paul-Ehrlich-Institute Langen, Germany.
Virology. 1996 May 15;219(2):367-75. doi: 10.1006/viro.1996.0262.
During the asymptomatic phase of human immunodeficiency virus 1 (HIV-1) infection the lymphatic tissues seem to function as a major reservoir of HIV. We have examined the viral load in peripheral blood mononuclear cells (PBMC) and lymph node mononuclear cells (LNMC) of 12 naturally and 4 experimentally long-term simian Immunodeficiency virus (SIV)-infected African green monkeys (AGM) to help explain the apathogenicity of the AGM isolates of SIV (SIVagm) in their natural host. The mean number of SIVagm producing cells determined by limiting dilution assay was found to be 1.7 +/- 2.2 and 2.1 +/- 3.3 per 10(5) PBMC or LNMC, respectively. Similarly, polymerase chain reaction analysis of serially diluted cells showed the mean provirus carrying cell number to be 2.8 +/- 3.7 per 10(5) PBMC and 4.0 +/- 5.5 per 10(5) LNMC. When normalized for CD4+ cells the provirus and infectious virus loads in the LNMC and PBMC were also similar. No trapping of virus particles could be detected by in situ hybridization or immunohistochemistry. The data demonstrate that in contrast to HIV-1-infected humans, the viral burden in the lymph nodes of long-term SIV(agm)-infected AGMs is comparable to that in the PBMC.
在人类免疫缺陷病毒1型（HIV-1）感染的无症状期，淋巴组织似乎是HIV的主要储存库。我们检测了12只自然感染和4只实验性长期感染猿猴免疫缺陷病毒（SIV）的非洲绿猴（AGM）外周血单个核细胞（PBMC）和淋巴结单个核细胞（LNMC）中的病毒载量，以帮助解释SIV非洲绿猴分离株（SIVagm）在其自然宿主中的无致病性。通过有限稀释法测定，每10⁵个PBMC或LNMC中产生SIVagm的细胞平均数分别为1.7±2.2和2.1±3.3。同样，对连续稀释细胞进行的聚合酶链反应分析显示，每10⁵个PBMC中携带前病毒的细胞平均数为2.8±3.7，每10⁵个LNMC中为4.0±5.5。以CD4⁺细胞进行标准化后，LNMC和PBMC中的前病毒和感染性病毒载量也相似。通过原位杂交或免疫组织化学未检测到病毒颗粒的滞留。数据表明，与HIV-1感染的人类不同，长期感染SIV（agm）的AGM淋巴结中的病毒载量与PBMC中的相当。
