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[甜菜变种(Beta vulgaris var. conditiva)的膳食纤维和果胶组分]

[Dietary fiber and pectin fractions of Beta vulgaris var. conditiva].

作者信息

Dongowski G

机构信息

Deutsches Institut für Ernährungsforschung Potsdam-Rehbrücke, Bergholz-Rehbrücke, Germany.

出版信息

Z Lebensm Unters Forsch. 1996 Apr;202(4):285-93. doi: 10.1007/BF01206098.

Abstract

The alcohol-insoluble substance (AIS) from red beet (Beta vulgaris var. conditiva Alef.) (3.31% of the edible substance) was extracted sequentially with water, ammonium oxalate, 0.05 N HCl and 0.05 N NaOH. Accordingly 3.93 g, about 0.8 g, 2.96 g resp. 3.80 g galacturonan/100 g AIS were extracted with this procedure. These pectin extracts were purified as Cu2 +-salts and fractionated into a water-soluble and a water-insoluble part. The composition of neutral monosaccharide units was estimated in the fractions. Gal, Ara and Glc dominated; Xyl, Rha and Man were also present but in smaller amounts. A higher GalA content was found in the soluble fractions (with the exception of the alkali extract). Pectins from red beet are middle- or low-esterified and partially acetylated. The composition of the AIS and of the residue after pectin extraction (RE) was determined (14.6 resp. 9.5% pectin; 10,6 resp. 17.6% protein; 58.7 resp. 64.9% total polysaccharides). In the AIS 23.3% soluble and 54.7% insoluble dietary fiber were estimated, whereas in RE 15.3 resp. 54.7% were found (enzymatic method). Following dietary fiber fractions were determined by the detergent method for both preparations: 39.0 resp. 52.7% NDF; 6.3 resp. 4.5% NDF filtrate; 23.6 resp. 41.8% ADF; 1.2 resp. 1.8% lignin. The water binding capacity decreased from 19.85 g water (AIS) to 11. 53 g water (RE) related to 1 g AUS. From these just 50% were found in the NDF fractions and about 13% in the ADF fractions. Alterations of the grown biological structures during pectin extraction and dietary fiber analysis (detergent method) were investigated by scanning electron microscopy.

摘要

从红甜菜(Beta vulgaris var. conditiva Alef.)中提取的醇不溶物(AIS)(占可食用物质的3.31%)依次用水、草酸铵、0.05N盐酸和0.05N氢氧化钠进行提取。相应地,用该方法分别提取出3.93g、约0.8g、2.96g和3.80g半乳糖醛酸聚糖/100g AIS。这些果胶提取物被纯化成为铜盐,并被分离成水溶性部分和水不溶性部分。对各部分中的中性单糖单元组成进行了估算。半乳糖(Gal)、阿拉伯糖(Ara)和葡萄糖(Glc)占主导;木糖(Xyl)、鼠李糖(Rha)和甘露糖(Man)也有存在,但含量较少。在可溶部分(碱提取物除外)中发现了较高的半乳糖醛酸(GalA)含量。红甜菜中的果胶为中酯化或低酯化且部分乙酰化。测定了AIS和果胶提取后残渣(RE)的组成(果胶含量分别为14.6%和9.5%;蛋白质含量分别为10.6%和17.6%;总多糖含量分别为58.7%和64.9%)。在AIS中,估计有23.3%的可溶性膳食纤维和54.7%的不溶性膳食纤维,而在RE中分别为15.3%和54.7%(酶法)。通过洗涤剂法测定了两种制剂中的膳食纤维组分:中性洗涤纤维(NDF)分别为39.0%和52.7%;NDF滤液分别为6.3%和4.5%;酸性洗涤纤维(ADF)分别为23.6%和41.8%;木质素分别为1.2%和1.8%。与1g AUS相关的水结合能力从19.85g水(AIS)降至11.53g水(RE)。其中,仅50%存在于NDF组分中,约13%存在于ADF组分中。通过扫描电子显微镜研究了果胶提取和膳食纤维分析(洗涤剂法)过程中生长生物结构的变化。

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