Hazlehurst D D, Hudson G, Sokol R J
National Blood Service, Trent Centre, Sheffield, UK.
Acta Haematol. 1996;95(2):112-6. doi: 10.1159/000203858.
An ELISA was developed for quantitating red-cell-bound IgG and IgA and its feasibility assessed on 50 blood donations and 32 clinical specimens with raised cell-bound IgG. Test and quality control samples and immunoglobulin standards (in red-cell lysate buffer) were assayed together. Calibration curves were derived from the standards, test values being read off and related to cell count. The working range was around 5-70 ng/ml, the upper limit being indefinitely extendable by dilution with lysate buffer. Ranges of results (and medians) in molecules per red cell were < 26-240 (40) for IgG and < 29-94 ( < 29) for IgA in the donations with 240-62,700 (1,200) for IgG and < 29-4,500 (73) for IgA in the clinical specimens. The method which can be adapted for any cell-bound immunoprotein should be particularly valuable in investigating autoimmune haemolysis.
开发了一种酶联免疫吸附测定法（ELISA）用于定量红细胞结合的IgG和IgA，并在50份献血样本和32份红细胞结合IgG升高的临床样本上评估了其可行性。测试样本、质量控制样本和免疫球蛋白标准品（在红细胞裂解缓冲液中）一起进行检测。根据标准品得出校准曲线，从校准曲线上读取测试值并与细胞计数相关联。工作范围约为5-70 ng/ml，上限可通过用裂解缓冲液稀释无限扩展。献血样本中，每红细胞分子的结果范围（及中位数）对于IgG为<26-240（40），对于IgA为<29-94（<29）；临床样本中，对于IgG为240-62,700（1,200），对于IgA为<29-4,500（73）。该方法可适用于任何细胞结合的免疫蛋白，在自身免疫性溶血的研究中应具有特别重要的价值。
