Kolenbrander P E, Hohman R J
J Bacteriol. 1977 Jun;130(3):1345-56. doi: 10.1128/jb.130.3.1345-1356.1977.
The whole cell ultrastructure during cell division and morphogenesis of Arthrobacter crystallopoietes was monitored using electron microscopic techniques. Glucose-grown spherical cells were inoculated into succinate-based medium. In this medium, the organism undergoes a morphogenetic cycle consisting of elongation of spheres to rods, exponential growth as rods, and fragmentation of rods to spherical cells. Raised bands or rings that encircled the cells were evident on the cell surface of both sphere- and rod-shaped cells. Many rod-shaped cells possessed two or more rings arranged adjacent to each other in a parallel orientation. At each cell division a new ring was formed on both siblings. However, as predicted by the proposed model of unidirectional cell growth and by maintaining a ring from the previous generation, unequal numbers of rings were observed on sibling cells. Only one ring was visible on most of the spherical inoculum cells, but in some cases a second ring perpendicular to the other ring was observed. Parallel rings were found on spherical cells resulting from fragmentation or reductive cell division of rods during the stationary growth phase. Thus, these spheres could be distinguished from inoculum spheres containing a single ring or perpendicular orientation of rings. The number of rings per cell and arrangement of rings on the cell surface of sibling cells after cell division, but before cell separation, are discussed with respect to cell age, cell division, and sphere-rod-sphere morphogenesis of A. crystallopoietes.
利用电子显微镜技术监测了产晶体节杆菌细胞分裂和形态发生过程中的全细胞超微结构。将葡萄糖培养的球形细胞接种到基于琥珀酸盐的培养基中。在这种培养基中，该生物体经历一个形态发生循环，包括球体伸长为杆状、杆状细胞指数生长以及杆状细胞破碎为球形细胞。在球形和杆状细胞的细胞表面都明显可见环绕细胞的凸起带或环。许多杆状细胞拥有两个或更多彼此相邻且平行排列的环。在每次细胞分裂时，两个子代细胞上都会形成一个新环。然而，正如所提出的单向细胞生长模型所预测的，并且由于保留了上一代的环，在子代细胞上观察到的环数量不相等。大多数球形接种细胞上只可见一个环，但在某些情况下会观察到与另一个环垂直的第二个环。在稳定生长期，由杆状细胞破碎或减数分裂产生的球形细胞上发现了平行环。因此，这些球体可以与含有单个环或环呈垂直排列的接种球体区分开来。本文讨论了产晶体节杆菌细胞分裂后但细胞分离前子代细胞的每个细胞上环的数量以及环在细胞表面的排列情况，涉及细胞年龄、细胞分裂和球体 - 杆状 - 球体形态发生。
