Takasaki Y, Yano T, Hirokawa K, Takeuchi K, Ando S, Takahashi T, Shimada K, Hashimoto H
Juntendo University School of Medicine, Tokyo, Japan.
Arthritis Rheum. 1996 May;39(5):855-62. doi: 10.1002/art.1780390519.
Epitopes on Ki antigen were analyzed using synthetic peptides, including KILT, a 16-mer peptide with an amino acid sequence homologous to the SV40 large T antigen nuclear localization signal (SV40 T NLS).
In addition to KILT, 4 synthetic peptides, all potential epitopes on Ki antigen according to computer analysis, were prepared and tested for reactivity with 49 anti-Ki-positive lupus sera by enzyme-linked immunosorbent assay.
Eighteen sera reacted with KILT, but not with other peptides. The reaction of anit-Ki sera with KILT was specifically inhibited by recombinant Ki antigen. Eight of 49 anti-Ki sera reacted with a 7-mer synthetic peptide of SV40 T NLS, and the reaction was specifically inhibited by KILT.
The 16-mer Ki peptide containing the sequence homologous to the SV40 T NLS is one of the antigenic epitopes recognized by anti-Ki antibodies in lupus sera.
使用合成肽分析Ki抗原上的表位，包括KILT，一种与SV40大T抗原核定位信号（SV40 T NLS）氨基酸序列同源的16肽。
除KILT外，还制备了4种合成肽，根据计算机分析它们均为Ki抗原上的潜在表位，并通过酶联免疫吸附试验检测其与49份抗Ki阳性狼疮血清的反应性。
18份血清与KILT反应，但不与其他肽反应。抗Ki血清与KILT的反应被重组Ki抗原特异性抑制。49份抗Ki血清中的8份与SV40 T NLS的一种7肽合成肽反应，且该反应被KILT特异性抑制。
含有与SV40 T NLS同源序列的16肽Ki肽是狼疮血清中抗Ki抗体识别的抗原表位之一。
