Chen H, McBroom D G, Zhu Y Q, Gold L, North T W
Department of Molecular, University of Colorado at Boulder 80309, USA.
Biochemistry. 1996 May 28;35(21):6923-30. doi: 10.1021/bi9600106.
High-affinity, high-specificity RNA ligands for reverse transcriptase from feline immunodeficiency virus (FIV) were isolated from an RNA library by the SELEX (Systematic Evolution of Ligands by EXponential enrichment) procedure. The selected RNA ligands bound to FIV reverse transcriptase with dissociation constants in the nanomolar range. One of the ligands was a potent inhibitor of the RNA-dependent DNA polymerase activity of both the recombinant and the virion-derived FIV reverse transcriptase. It also inhibited the reverse transcriptase from an FIV mutant that is resistant to 3'-azido-3'-deoxythymidine (AZT). The inhibition of FIV reverse transcriptase was competitive with respect to template-primer and noncompetitive with respect to deoxyribonucleoside 5'-triphosphates. This ligand was specific for the FIV enzyme and did not inhibit other reverse transcriptases tested (avian myeloblastosis virus, Moloney murine leukemia virus, and human immunodeficiency virus type 1).
通过指数富集配体系统进化（SELEX）程序，从RNA文库中分离出了针对猫免疫缺陷病毒（FIV）逆转录酶的高亲和力、高特异性RNA配体。所选的RNA配体与FIV逆转录酶结合，解离常数在纳摩尔范围内。其中一种配体是重组和病毒体来源的FIV逆转录酶的RNA依赖性DNA聚合酶活性的有效抑制剂。它还抑制了对3'-叠氮基-3'-脱氧胸苷（AZT）耐药的FIV突变体的逆转录酶。FIV逆转录酶的抑制作用在模板引物方面是竞争性的，而在脱氧核糖核苷5'-三磷酸方面是非竞争性的。这种配体对FIV酶具有特异性，不抑制所测试的其他逆转录酶（禽成髓细胞瘤病毒、莫洛尼鼠白血病病毒和人类免疫缺陷病毒1型）。
