Schwedt G, Bussemas H H, Lippmann C
J Chromatogr. 1977 May 1;143(3):259-66.
The possibilities were examined for the high-pressure liquid chromatographic analysis of cortisol with methods of adsorption, distribution and reversed-phase chromatography. Free cortisol in urine can be determined by extraction with choloroform and subsequent adsorption chromatography on silica gel with a mobile phase consisting of 1.5% methanol and 0.2% water in chloroform. The time needed for this chromatographic analysis is 10--15 min; the limit of determination is 3 ng of cortisol for one injection.
研究了采用吸附、分配和反相色谱法对皮质醇进行高压液相色谱分析的可能性。尿中游离皮质醇可通过用氯仿萃取，随后在硅胶上进行吸附色谱分析来测定，流动相为氯仿中含1.5%甲醇和0.2%水。这种色谱分析所需时间为10 - 15分钟；一次进样的测定限为3纳克皮质醇。
