Rubnitz J E, Behm F G, Curcio-Brint A M, Pinheiro R P, Carroll A J, Raimondi S C, Shurtleff S A, Downing J R
Department of Hematology/Oncology, St. Jude Children'sResearch Hospital, Memphis, TN 38105, USA.
Blood. 1996 Jun 1;87(11):4804-8.
MLL is fused to ENL or ELL in acute leukemias that contain t(ll;19)(q23;p13). Although ENL and ELL localize to chromosome 19, bands p13.3 and p13.1, respectively, these breakpoints are not always readily distinguished by standard cytogenetics. We therefore used reverse transcriptase-polymerase chain reaction (RT-PCR) assays to analyze 26 cases of childhood acute leukemia containing t(11;19) to determine the frequencies of ENL and ELL involvement. All 17 cases of acute lymphoblastic leukemia (ALL) had MLL/ENL fusion transcripts. By contrast, of the 9 cases of acute myeloid leukemia (AML) analyzed, 6 had MLL/ENL fusions, 2 had MLL/ELL fusions, and 1 case had no RT-PCR-detectable MLL fusion mRNA. These data suggest that the majority of 11;19 translocations involve ENL, whereas involvement of ELL is relatively uncommon in childhood acute leukemia and may be restricted to AML.
在含有t(11;19)(q23;p13)的急性白血病中，MLL与ENL或ELL融合。尽管ENL和ELL分别定位于19号染色体的p13.3和p13.1带，但这些断点通过标准细胞遗传学并不总是容易区分。因此，我们使用逆转录聚合酶链反应（RT-PCR）分析了26例含有t(11;19)的儿童急性白血病病例，以确定ENL和ELL受累的频率。所有17例急性淋巴细胞白血病（ALL）均有MLL/ENL融合转录本。相比之下，在分析的9例急性髓细胞白血病（AML）中，6例有MLL/ENL融合，2例有MLL/ELL融合，1例未检测到RT-PCR可检测的MLL融合mRNA。这些数据表明，大多数11;19易位涉及ENL，而ELL的受累在儿童急性白血病中相对不常见，可能仅限于AML。
