Robert B, Malassiné A, Bourgeois C, Mignot T M, Cronier L, Ferré F, Duc-Goiran P
INSERM U.361, Université René Descartes, Maternité Baudelocque, Paris, France.
Eur J Endocrinol. 1996 Apr;134(4):490-6. doi: 10.1530/eje.0.1340490.
We have shown previously the presence of immunoreactive endothelin in cultured trophoblastic cells from human term placenta as well as in the trophoblast-conditioned medium. To confirm whether or not the differentiated syncytiotrophoblast is a site for endothelin synthesis, we investigated, by reverse transcription and polymerase chain reaction, the expression of the three preproendothelin genes in 3-day cultured trophoblast. While no endothelin-2 precursor mRNA was detected, preproendothelin-1 mRNA was found to be expressed by the trophoblast. The endothelin-3 precursor gene was also expressed, but at low level and it was detected only after Southern blotting and oligonucleotide hybridization. The ability of trophoblast in culture to express the endothelin precursor genes supports the idea that, in human term placenta, villous syncytiotrophoblast that lines the intervillous space containing maternal blood acts as an endothelial layer.
我们之前已经表明，在来自足月人胎盘的培养滋养层细胞以及滋养层条件培养基中存在免疫反应性内皮素。为了证实分化的合体滋养层是否为内皮素合成的场所，我们通过逆转录和聚合酶链反应研究了3天培养的滋养层中三种前内皮素原基因的表达。虽然未检测到内皮素-2前体mRNA，但发现滋养层表达前内皮素-1 mRNA。内皮素-3前体基因也有表达，但水平较低，仅在Southern印迹和寡核苷酸杂交后才检测到。培养的滋养层表达内皮素前体基因的能力支持了这样一种观点，即在足月人胎盘中，衬于含有母体血液的绒毛间隙的绒毛合体滋养层起着内皮细胞层的作用。
