Mao L, Lee J S, Fan Y H, Ro J Y, Batsakis J G, Lippman S, Hittelman W, Hong W K
Department of Thoracic/Head and Neck Medical Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Nat Med. 1996 Jun;2(6):682-5. doi: 10.1038/nm0696-682.
To better understand genetic alterations in oral premalignant lesions, we examined 84 oral leukoplakia samples from 37 patients who had been enrolled in a chemoprevention trial. The samples were analyzed for two microsatellite markers located at chromosomes 9p21 and 3p14. Loss of heterozygosity (LOH) at either or both loci was identified in 19 of the 37 (51%) patients. Of these 19 patients, seven (37%) have developed head and neck squamous cell carcinoma (HNSCC) while only one of 18 (6%) of patients without LOH developed HNSCC. Our data suggest that clonal genetic alterations are common in oral premalignant lesions; that multiple genetic alterations have already occurred in oral premalignant lesions, allowing at least a focal clonal expansion; and that losses of the 9p21 and 3p14 regions may be related to early processes of tumorigenesis in HNSCC. These genetic alterations in premalignant tissues may serve as markers for cancer risk assessment.
为了更好地了解口腔癌前病变中的基因改变，我们检测了来自37名参与化学预防试验患者的84份口腔白斑样本。对位于9号染色体p21区域和3号染色体p14区域的两个微卫星标记进行了分析。在37名患者中的19名（51%）患者中，发现一个或两个位点存在杂合性缺失（LOH）。在这19名患者中，7名（37%）已经发生头颈部鳞状细胞癌（HNSCC），而在18名无LOH的患者中只有1名（6%）发生HNSCC。我们的数据表明，克隆性基因改变在口腔癌前病变中很常见；口腔癌前病变中已经发生了多种基因改变，至少允许局部克隆性扩增；9号染色体p21区域和3号染色体p14区域的缺失可能与HNSCC的早期肿瘤发生过程有关。癌前组织中的这些基因改变可作为癌症风险评估的标志物。
