人氧化型低密度脂蛋白中3β-羟基-5α-胆甾-6-烯-5-氢过氧化物的鉴定
Identification of 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide in human oxidized LDL.
作者信息
Caruso D, Rasetti M F, De Angelis L, Galli G
机构信息
Institute of Pharmacological Sciences, Milan, Italy.
出版信息
Chem Phys Lipids. 1996 Mar 29;79(2):181-6. doi: 10.1016/0009-3084(95)02519-7.
PMID:8640903
Abstract
Oxidized LDL exhibits cytotoxic activity towards endothelial cells, which is thought to be mediated by the products derived also from cholesterol oxidation, mainly involving the B-ring. By LC-PB-EI-MS and EI-MS/MS analysis, we have identified 3 beta-hydroxy-5 alpha-cholest-6-ene-5-hydroperoxide among the cholesterol oxidation products in LDL incubated with micromolar concentrations of copper ions.
摘要
氧化型低密度脂蛋白对内皮细胞具有细胞毒性活性，这种活性被认为是由同样来源于胆固醇氧化的产物介导的，主要涉及B环。通过液相色谱-大气压化学电离-电子轰击质谱（LC-PB-EI-MS）和串联电子轰击质谱（EI-MS/MS）分析，我们在与微摩尔浓度铜离子孵育的低密度脂蛋白的胆固醇氧化产物中鉴定出了3β-羟基-5α-胆甾-6-烯-5-氢过氧化物。
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