非特异性醇解，一种人类免疫缺陷病毒1型及其他逆转录病毒整合酶的新型内切核酸酶活性。
Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases.
作者信息
Katzman M, Sudol M
机构信息
Department of Medicine, The Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033-0850, USA.
出版信息
J Virol. 1996 Apr;70(4):2598-604. doi: 10.1128/JVI.70.4.2598-2604.1996.
Abstract
Retroviral integrase (IN) exhibits a previously unrecognized endonuclease activity which we have termed nonspecific alcoholysis. This action occurred at every position in nonviral DNA sequences except those near 5' ends and is clearly distinguished from, and was not predicted by, the site-specific alcoholysis activity previously described for IN at the processing site near viral DNA termini. The integrases of human immunodeficiency virus type 1, visna virus, and Rous sarcoma virus exhibited different target site preferences in this new assay. The isolated central domain of human immunodeficiency virus type 1 IN preferred the same sites as the full-length protein. Nonspecific alcoholysis may provide insights into the structure and function of IN and other endonucleases and suggests that stimulators of some activities possessed by retroviral enzymes should be sought as antiviral agents.
摘要
逆转录病毒整合酶（IN）表现出一种以前未被认识的核酸内切酶活性，我们将其称为非特异性醇解。这种作用发生在非病毒DNA序列的每个位置，除了靠近5'端的那些位置，并且与先前在病毒DNA末端附近的加工位点描述的IN的位点特异性醇解活性明显不同，且未被其预测。在这项新试验中，人类免疫缺陷病毒1型、维斯纳病毒和劳氏肉瘤病毒的整合酶表现出不同的靶位点偏好。人类免疫缺陷病毒1型IN分离出的中央结构域与全长蛋白偏好相同的位点。非特异性醇解可能为IN和其他核酸内切酶的结构和功能提供见解，并表明应寻找逆转录病毒酶所具有的某些活性的刺激剂作为抗病毒药物。
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