Hotchkiss T L, Hollingsworth M J
SUNY at Buffalo 14260, USA.
Nucleic Acids Symp Ser. 1995(33):207-8.
The spinach chloroplast large ATP synthase cluster encodes five genes, the last four of which encode chloroplast ATP synthase subunits. Because the stoichiometry of the ATP synthase polypeptide products differs substantially from that of the primary transcripts, we have chosen this system as a model to study posttranscriptional mechanisms that regulate chloroplast gene expression. Here we present data on a chloroplast extract containing factors that bind to the 5' untranslated regions of chloroplast ATP synthase transcripts. The binding is transcript-specific. This binding may influence translational efficiency of chloroplast transcripts.
菠菜叶绿体大型ATP合酶簇编码五个基因，其中后四个基因编码叶绿体ATP合酶亚基。由于ATP合酶多肽产物的化学计量与初级转录本的化学计量有很大差异，我们选择这个系统作为模型来研究调节叶绿体基因表达的转录后机制。在这里，我们展示了关于一种叶绿体提取物的数据，该提取物含有与叶绿体ATP合酶转录本5'非翻译区结合的因子。这种结合具有转录特异性。这种结合可能会影响叶绿体转录本的翻译效率。
