Endo H, Ueno E, Ichida M, Tsukamoto T, Hamamoto T, Kagawa Y
Department of Biochemistry, Jichi Medical School, Tochigi, Japan.
Nucleic Acids Symp Ser. 1995(33):30-2.
The human Ras-related nuclear protein Ran/TC4 is the member of a well conserved family of GTPases that can regulate cell-cycle progression, nuclear structure, protein import, and RNA export through nuclear pore complex (NPC). Translocation of RNA through the NPC also needs a RNA-binding protein as a possible mediator of export. By a low-stringency hybridization with a PCR fragment encoding RNA-binding domain as a probe, we obtained a partial human cDNA from skeletal muscle cDNA library. The deduced amino acid sequence indicated that the gene possessed both RNA-binding domain and Ran-binding domain, therefore it is a candidate gene for RNA export carrier protein. RNA blot analysis showed that this gene appeared approximately 8-10 kilo base in length and that the gene expression level in several human tissues is ubiquitous. The function of the gene will be discussed.
人类Ras相关核蛋白Ran/TC4是GTP酶中一个高度保守家族的成员，该家族可通过核孔复合体（NPC）调节细胞周期进程、核结构、蛋白质输入和RNA输出。RNA通过NPC的转运也需要一种RNA结合蛋白作为可能的输出介质。通过用编码RNA结合结构域的PCR片段作为探针进行低严谨度杂交，我们从骨骼肌cDNA文库中获得了一个部分人类cDNA。推导的氨基酸序列表明该基因同时拥有RNA结合结构域和Ran结合结构域，因此它是RNA输出载体蛋白的候选基因。RNA印迹分析表明该基因长度约为8 - 10千碱基，且在几种人类组织中的基因表达水平普遍存在。将对该基因的功能进行讨论。
